The book covers all of the major topics in bioinformatics, and touches on several of the minor ones. There are 5 long chapters:
Chapter 1 Introduction: introduces the basics of the field, describing the basics of data archiving, the WWW, computers and computer programming, biological classification and nomenclature, phylogenetic relationships and use of sequences, PSI-BLAST, and protein structure.
Chapter 2 Genome organization and evolution: genomics and proteomics, methods of genetic information transmission, genes and genomes, SNPs, genome evolution.
Chapter 3 Archives and information retrieval: this contains a detailed discussion of various databases and how to interact with them.
Chapter 4 Alignments and phylogenetic trees: this vast majority of this chapter covers many aspects of the important area of sequence alignment, including BLAST and HMMs. Then it has short sections on phylogeny and phylogenetic trees, again covering the basics.
Chapter 5 Protein structure and drug discovery: this starts with protein folding, and deals with hydrophobicity, structural alignments, DALI, and then evolution, classification and prediction of protein structures and function. Finally it touches on drug discovery in this context.

One of the nice things about this book is the code samples, written in the bioinformatician's favorite language, Perl. These are printed and discussed in the book, but then also available on the web site that is associated with the book, so you don't have to type it in yourself. In addition to the programs, the website also has graphics from the book, many of which rotate so you can see them from different positions (can't get that in a book!). It also has the web links mentioned in the book, so you can explore them more conveniently than having to flip through the book and type the URLs in.

Structure contains many examples of enzymatic function to describe the various processes involved in enzyme specificity and mechanism that the reader will find useful without being overwhelming, unlike so many other Biochemistry textbooks. It appears that Fersht has selected his examples to explain a point rather than to be a comprehensive overview of catalysis. In addition, there are very useful accounts for the researcher in how to obtain such information by the way of applying the model or equations given to a real-life system.

In addition to the enzymatic structure and function that constitutes the bulk of the book, there is a concise account of Protein Engineering for both enzyme function study and protein stability itself. Fersht traverses the field from the fundamentals to current theory on folding pathways, illuminating the key concepts and experimental methods on the way. The textbook concludes many of the chapters with several case studies illustrating the methods used and information gleaned by real research and this section is particularly good on folding pathways for proteins. However, it is perhaps too brief for a researcher in protein folding and Roger Pains Mechanisms of Protein Folding is a more comprehensive source for the graduate of lecturer.

In conclusion, this is a comprehensive account of enzyme structure and mechanism from a physical first-principles point of view. It is not a book recommended for a reader seeking a brief introduction, nor for a graduate seeking a deeper insight into protein folding than basic methods for analysis. That said, it is the only textbook that combines these related disciplines in a clear manner. It is easy to read, if a little curt in parts, but it is difficult not to be impressed by the sound and detailed account of enzymes from a mechanistic background.